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51.
Bruno Studer Torben Asp Ursula Frei Stephan Hentrup Helena Meally Aurélie Guillard Susanne Barth Hilde Muylle Isabel Roldán-Ruiz Philippe Barre Carole Koning-Boucoiran Gerda Uenk-Stunnenberg Oene Dolstra Leif Skøt Kirsten P. Skøt Lesley B. Turner Mervyn O. Humphreys Roland Kölliker Niels Roulund Klaus K. Nielsen Thomas Lübberstedt 《Molecular breeding : new strategies in plant improvement》2008,21(4):533-548
An expressed sequence tag (EST) library of the key grassland species perennial ryegrass (Lolium perenne L.) has been exploited as a resource for microsatellite marker development. Out of 955 simple sequence repeat (SSR) containing
ESTs, 744 were used for primer design. Primer amplification was tested in eight genotypes of L. perenne and L. multiflorum representing (grand-) parents of four mapping populations and resulted in 464 successfully amplified EST-SSRs. Three hundred
and six primer pairs successfully amplified products in the mapping population VrnA derived from two of the eight genotypes
included in the original screening and revealed SSR polymorphisms for 143 ESTs. Here, we report on 464 EST-derived SSR primer
sequences of perennial ryegrass established in laboratory assays, providing a dedicated tool for marker assisted breeding
and comparative mapping within and among forage and turf grasses.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
52.
Nagotu S Krikken AM Otzen M Kiel JA Veenhuis M van der Klei IJ 《Traffic (Copenhagen, Denmark)》2008,9(9):1471-1484
We show that Mdv1 and Caf4, two components of the mitochondrial fission machinery in Saccharomyces cerevisiae , also function in peroxisome proliferation. Deletion of MDV1 , CAF4 or both, however, had only a minor effect on peroxisome numbers at peroxisome-inducing growth conditions, most likely related to the fact that Vps1 – and not Dnm1 – is the key player in peroxisome fission in this organism. In contrast, in Hansenula polymorpha , which has only a Dnm1-dependent peroxisome fission machinery, deletion of MDV1 led to a drastic reduction of peroxisome numbers. This phenotype was accompanied by a strong defect in mitochondrial fission. The MDV1 paralog CAF4 is absent in H. polymorpha . In wild-type H. polymorpha , cells Dnm1–mCherry and green fluorescent protein (GFP)–Mdv1 colocalize in spots that associate with both peroxisomes and mitochondria. Furthermore, Fis1 is essential to recruit Mdv1 to the peroxisomal and mitochondrial membrane. However, formation of GFP–Mdv1 spots – and related to this normal organelle fission – is strictly dependent on the presence of Dnm1. In dnm1 cells, GFP–Mdv1 is dispersed over the surface of peroxisomes and mitochondria. Also, in H. polymorpha mdv1 or fis1 cells, the number of Dnm1–GFP spots is strongly reduced. These spots still associate to organelles but are functionally inactive. 相似文献
53.
The acyclic CB1R inverse agonist taranabant mediates weight loss by increasing energy expenditure and decreasing caloric intake 总被引:6,自引:0,他引:6
Addy C Wright H Van Laere K Gantz I Erondu N Musser BJ Lu K Yuan J Sanabria-Bohórquez SM Stoch A Stevens C Fong TM De Lepeleire I Cilissen C Cote J Rosko K Gendrano IN Nguyen AM Gumbiner B Rothenberg P de Hoon J Bormans G Depré M Eng WS Ravussin E Klein S Blundell J Herman GA Burns HD Hargreaves RJ Wagner J Gottesdiener K Amatruda JM Heymsfield SB 《Cell metabolism》2008,7(1):68-78
Cannabinoid 1 receptor (CB1R) inverse agonists are emerging as a potential obesity therapy. However, the physiological mechanisms by which these agents modulate human energy balance are incompletely elucidated. Here, we describe a comprehensive clinical research study of taranabant, a structurally novel acyclic CB1R inverse agonist. Positron emission tomography imaging using the selective CB1R tracer [(18)F]MK-9470 confirmed central nervous system receptor occupancy levels ( approximately 10%-40%) associated with energy balance/weight-loss effects in animals. In a 12-week weight-loss study, taranabant induced statistically significant weight loss compared to placebo in obese subjects over the entire range of evaluated doses (0.5, 2, 4, and 6 mg once per day) (p < 0.001). Taranabant treatment was associated with dose-related increased incidence of clinical adverse events, including mild to moderate gastrointestinal and psychiatric effects. Mechanism-of-action studies suggest that engagement of the CB1R by taranabant leads to weight loss by reducing food intake and increasing energy expenditure and fat oxidation. 相似文献
54.
Lambrechts A Gevaert K Cossart P Vandekerckhove J Van Troys M 《Trends in cell biology》2008,18(5):220-227
Listeria monocytogenes is a master of mimicry that uses the host cell actin system both to move within the cytoplasm of infected cells and for cell-to-cell spread. Recent studies of Listeria and similarly acting pathogens have generated leaps in our understanding of the actin-based force producing machinery. This machinery is essential for most motile properties of cells, not least for cell migration. In a minimal configuration, it consists of the Arp2/3-complex, Ena-VASP proteins, cofilin, capping protein and a nucleation-promoting factor. In this review, we discuss current models of pseudopodial protrusions and describe how the road to more complex models lies open and is already paved by recent studies using Listeria-based biomimetic motility assays. 相似文献
55.
56.
Sander A. Peters Erwin Datema Dóra Szinay Marjo J. van Staveren Elio G.W.M. Schijlen Jan C. van Haarst Thamara Hesselink Marleen H.C. Abma-Henkens Yuling Bai Hans de Jong Willem J. Stiekema René M. Klein Lankhorst Roeland C.H.J. van Ham 《The Plant journal : for cell and molecular biology》2009,58(5):857-869
57.
Joana RF Abreu Daphne de Launay Marjolein E Sanders Aleksander M Grabiec G Marleen van de Sande Paul P Tak Kris A Reedquist 《Arthritis research & therapy》2009,11(4):R121-13
Introduction
Fibroblast-like synoviocytes (FLS) from rheumatoid arthritis (RA) patients share many similarities with transformed cancer cells, including spontaneous production of matrix metalloproteinases (MMPs). Altered or chronic activation of proto-oncogenic Ras family GTPases is thought to contribute to inflammation and joint destruction in RA, and abrogation of Ras family signaling is therapeutic in animal models of RA. Recently, expression and post-translational modification of Ras guanine nucleotide releasing factor 1 (RasGRF1) was found to contribute to spontaneous MMP production in melanoma cancer cells. Here, we examine the potential relationship between RasGRF1 expression and MMP production in RA, reactive arthritis, and inflammatory osteoarthritis synovial tissue and FLS. 相似文献58.
Communication is an important aspect of human life, allowing us to powerfully coordinate our behaviour with that of others. Boiled down to its mere essentials, communication entails transferring a mental content from one brain to another. Spoken language obviously plays an important role in communication between human individuals. Manual gestures however often aid the semantic interpretation of the spoken message, and gestures may have played a central role in the earlier evolution of communication. Here we used the social game of charades to investigate the neural basis of gestural communication by having participants produce and interpret meaningful gestures while their brain activity was measured using functional magnetic resonance imaging. While participants decoded observed gestures, the putative mirror neuron system (pMNS: premotor, parietal and posterior mid-temporal cortex), associated with motor simulation, and the temporo-parietal junction (TPJ), associated with mentalizing and agency attribution, were significantly recruited. Of these areas only the pMNS was recruited during the production of gestures. This suggests that gestural communication relies on a combination of simulation and, during decoding, mentalizing/agency attribution brain areas. Comparing the decoding of gestures with a condition in which participants viewed the same gestures with an instruction not to interpret the gestures showed that although parts of the pMNS responded more strongly during active decoding, most of the pMNS and the TPJ did not show such significant task effects. This suggests that the mere observation of gestures recruits most of the system involved in voluntary interpretation. 相似文献
59.
Melanie Zaparty Dominik Esser Susanne Gertig Patrick Haferkamp Theresa Kouril Andrea Manica Trong K. Pham Julia Reimann Kerstin Schreiber Pawel Sierocinski Daniela Teichmann Marleen van Wolferen Mathias von Jan Patricia Wieloch Sonja V. Albers Arnold J. M. Driessen Hans-Peter Klenk Christa Schleper Dietmar Schomburg John van der Oost Phillip C. Wright Bettina Siebers 《Extremophiles : life under extreme conditions》2010,14(1):119-142
60.
Veerle Lejon Dieudonné Mumba Ngoyi Marleen Boelaert Philippe Büscher 《PLoS neglected tropical diseases》2010,4(1)